ISOLATION AND SERUM-FREE CULTURE OF EPITHELIAL CELLS DERIVED FROM EPITHELIAL RESTS OF MALASSEZ IN HUMAN PERIODONTAL LIGAMENT

2000 ◽  
Vol 36 (8) ◽  
pp. 548 ◽  
Author(s):  
TAKENORI YAMANAKA ◽  
AKIHIKO SAKAMOTO ◽  
YOSHIHARU TANAKA ◽  
YAN ZHANG ◽  
YASUTAKA HAYASHIDO ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Periodontal Ligament ◽  
Serum Free ◽  
Epithelial Rests Of Malassez

Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology

1991 ◽  
Vol 125 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Bertil G. Casslén ◽  
Michael J. K. Harper
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Collagen Matrix ◽  
Human Endometrium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Endometrial Stromal Cells ◽  
Prolonged Incubation ◽  
Serum Free ◽  
Endometrial Epithelial Cells

Abstract. The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2α (PGF2α) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2α by epithelial cells. We conclude that the secretion of PGF2α from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.

Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase

1985 ◽  
Vol 5 (12) ◽  
pp. 1071-1077 ◽  
Author(s):  
Geoffrey A. Stevenson ◽  
J. Guy Lyons ◽  
David A. Cameron ◽  
Robert L. O'Grady
Keyword(s):  
Epithelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mammary Carcinoma ◽  
Bovine Serum ◽  
Defined Medium ◽  
Carcinoma Cells ◽  
Serum Free ◽  
Vertebrate Collagenase

Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase. This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.

Characterization of growth and differentiation of normal human submandibular gland epithelial cells in a serum-free medium

1993 ◽  
Vol 54 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Masaya Okura ◽  
Kanemitsu Shirasuna ◽  
Tsutomu Hiranuma ◽  
Hideo Yoshioka ◽  
Hirokazu Nakahara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Submandibular Gland ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Normal Human
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